Abstract: The recent spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated Coronavirus disease (COVID-19), has gripped the entire international community and has caused widespread public health concerns. Despite global efforts to contain the disease spread, the outbreak is still on a rise owing to the community spread pattern of this infection. This is a zoonotic infection that, similar to other coronavirus infections, is believed to have been originated in bats and pangolins, and later transmitted to humans. Once in the human body, this Corona virus (SARS-CoV-2) is abundantly present in nasopharyngeal and salivary secretions of affected patients and its spread is predominantly thought to be respiratory droplet/contact in nature. Dental professionals, including endodontists, may soon encounter patients with suspected or confirmed SARS-CoV-2 infection and will have to act diligently not only to provide care but at the same time prevent nosocomial spread of infection. Thus, the aim of this article is to provide a brief overview of the epidemiology, symptoms and routes of transmission of this novel infection. In addition, specific recommendations for dental practice are suggested for patient screening, infection control strategies and patient management protocol. Clinical Relevance Dental care providers need to be aware and prepared for tackling any impending infectious disease challenge as might be the case in the current outbreak of SARS-Cov-2 transmission and its associated Coronavirus disease (COVID-19) that can be life-threatening to susceptible patients. Keywords coronavirus, COVID-19, SARS-CoV-2, virus, nosocomial, disease transmission, infection, dentistry, endodontics Introduction The outbreak of coronavirus disease 2019 (COVID-19) in the area of Wuhan, China has evolved rapidly into a public health crisis (1) and has spread exponentially to other parts of the world. The novel coronavirus belongs to a family of single-stranded RNA viruses known as Coronaviridae (2). This family of viruses are known to be zoonotic or transmitted from animals to humans. These include severe acute respiratory syndrome coronavirus (SARS-CoV), first identified in 2002 and the Middle East respiratory syndrome coronavirus (MERS-CoV), first identified in 2012 (3). There is strong evidence that this novel coronavirus has similarity to coronavirus species found in bats and potentially pangolins, confirming the zoonotic nature of this new cross-species viral-mediated disease (4,5). Since the published genome sequence for this novel coronavirus has a close resemblance with other ß-Coronaviruses such as SARS-CoV and MERS-CoV, the Coronavirus Study Group of the International Committee on Taxonomy of Viruses has given its scientific name as SARS-CoV-2, even though it is popularly called COVID-19 virus (6,7). On January 30, 2020, the World Health Organization (WHO) declared the rampant spread of SARS-CoV-2 and its associated disease (COVID-19) a public health emergency with a currently known overall mortality rate to be as high as 3.4% (8,9). According to WHO situation report (March 16, 2020) update on COVID-19, there have been more than 160,000 reported cases and 6,000 deaths worldwide (10) and this number continues to increase. Therefore, measures for prevention, identification and management must be in place for appropriate mitigation of further spread. Given the widespread transmission of SARS-CoV-2 and reports of its spread to Health Care Providers (HCPs) (3,11), dental professionals are at high risk for nosocomial infection and can become potential carriers of the disease. Such risks can be attributed to the unique nature of dental interventions, which include aerosol generation, handling of sharps and proximity of the provider to the patient’s oropharyngeal region. In addition, if adequate precautions are not taken, the dental office can potentially expose patients to cross-contamination. As the understanding of this novel disease is evolving, dental practices should be better prepared to identify a possible COVID-19 infection, and refer patients with suspected, confirmed, or a history of COVID-19 infection to appropriate treatment centers. Here, we summarize current recommendations for diagnosing and managing patients with COVID-19. While this information is current up to March 2020. Symptoms Patients with COVID-19 usually present with clinical symptoms of fever, cough and myalgia. In addition, abnormal chest X-Ray and computer tomography (CT) findings such as ground-glass opacities are typically found in the chest (12). Notably, 80% of these patients have only mild symptoms that resemble flu-like symptoms and seasonal allergies, which might lead to an increased number of undiagnosed cases (13). Although SARS-CoV-2 is known to be highly transmissible when patients are most symptomatic, it is noteworthy that the incubation period can range from 0-24 days, therefore transmission can occur before any symptoms are apparent (12,14). Severe forms of this disease have a predilection for males with a mean age of 56 years with pre-existing chronic illnesses such as cardiovascular disease or immunosuppression. The higher risk patient population manifests symptoms typical of pneumonia or acute respiratory distress syndrome (12). Routes of transmission SARS-Cov-2 infections typically spread through respiratory droplets or by contact (1). Therefore, coughing or sneezing by an infected person can render SARS-CoV-2 airborne, potentially infecting individuals in close contact (radius of approximately 6 feet). This had led to recommendations of social distancing. Another important route of transmission is if droplets of SARS-CoV-2 land on inanimate objects located nearby an infected individual and are subsequently touched by other individuals (1). Indeed, a recent report suggests that the virus remains viable for up to 9 days when it is on a hard surface such as plastic or metal. Thus, disinfection of objects and hand washing is essential for halting the spread of this disease. This recommendation is strengthened considering that people touch their face on an average 23 times per hour, with 44% of these occurrences involving the mucous membranes of mouth and/or nose (15). In addition, studies have shown the presence of SARS-CoV-2 in both saliva and feces of the affected patients (16,17). It is known that SARS-CoV-2 can bind to human angiotensin converting enzyme 2 (ACE-2) positive cells, which are highly concentrated in salivary glands; this may be a possible explanation for the presence of SARS-CoV-2 in secretory saliva (18,19). Therefore, there is a potential for transmission of COVID-19 via aerosol, fomites or fecal-oral route that may contribute to nosocomial spread in the dental office setting (20). Patient management and prevention of nosocomial infection Based on the experience gained from the previous outbreak of SARS-CoV and data available on SARS-CoV-2 and its associated disease (COVID-19), certain specific measures are discussed here for dental patient management in this epidemic period of COVID-19. On March 16, 2020, the American Dental Association recommended that dentists postpone elective procedures for the next three weeks and instead only provide treatment for dental emergencies. I. TELE-SCREENING AND TRIAGING: Initial screening via telephone to identify patients with suspected or possible COVID-19 infection can be performed remotely at the time of scheduling appointments. The two most pertinent questions for initial screening should include any travel history to COVID-19 affected areas and the presence of any febrile respiratory illness symptoms such as fever and cough. Importantly, to identify high risk areas, live global tracking of reported cases can be done using the dashboard made accessible by the Center for Systems Science and Engineering (CSSE) at Johns Hopkins University (21). Positive response to either of the two questions should raise initial concern and elective dental care should be deferred for at least two weeks (Note: As mentioned previously, the incubation period for SARS-CoV-2 is variable and can range from 0-24 days). Patients should be encouraged to be in self quarantine particularly if they have been to areas considered at high risk for infections (22). II. PATIENT EVALUATION & COHORTING Upon patient arrival in dental practice, patients should complete a detailed medical history form, COVID-19 screening questionnaire and assessment of a true emergency questionnaire. Dental professionals should measure the patient’s body temperature using a non-contact forehead thermometer or with cameras having infrared thermal sensors (20). Patients who present with fever (>100.4°F = 38°C) and/or respiratory disease symptoms, should have elective dental care deferred for at least 2-3 weeks. As per the Centers for Disease Control and Prevention (CDC) guidelines, individuals with suspected COVID-19 infection should be seated in a separate, well-ventilated waiting area at least 6 feet from the unaffected patients seeking care (23). Patients should be requested to wear a surgical mask and follow proper respiratory hygiene, such as covering the mouth and nose with a tissue before coughing and sneezing, and then discarding the tissue (23). After informing the patients to self-quarantine themselves, dentists should instruct the patients to report to their physician to rule out possibility of COVID-19. Pharmacologic management: In suspected or confirmed cases of COVID-19 infections, patients requiring urgent dental care for conditions such as tooth pain and/or swelling, pharmacological management in the form of antibiotics and/or analgesics is an alternative. This approach may offer symptomatic relief and will provide dental professionals some time to develop a plan to deliver dental care with all appropriate measures in place to prevent the spread of infection. III. SPECIFIC DENTAL TREATMENT RECOMMENDATIONS Patients with active febrile and respiratory illness will most likely not present to dental practices. Based on the assessment of emergency questionnaire, clinicians can gauge the severity of the dental condition and make an informed decision to either provide or defer dental care. Certain instances such as dentoalveolar trauma or progressive fascial space infection will definitely warrant emergency dental intervention. In the unlikely event of providing dental care to suspected or confirmed cases of COVID-19 infection, dentists should be cognizant of the following recommendations: Dentists should follow standard, contact, and airborne precautions including the appropriate use of personal protective equipment (PPE) and hand hygiene practices. Preprocedural mouth rinse: Previous studies have shown that SARS and MERS were highly susceptible to povidone mouth rinse. Therefore, preprocedural mouth rinse with 0.2% povidone-iodine might reduce the load of corona viruses in saliva. Use of disposable (single use) devices such as mouth mirror, syringes and blood pressure cuff to prevent cross-contamination. Radiographs: Extraoral imaging such as panoramic radiograph or CBCT should be used to avoid the gag reflex or cough that may occur with intraoral imaging. When intraoral imaging is mandated, sensors should be double barriered to prevent perforation and cross-contamination. Dentists should use a rubber dam to minimize splatter generation (of course, this is the standard of care for non-surgical endodontic treatment). It may be advantageous to place the rubber dam so that it covers the nose. The actual procedure should minimize generation of aerosol. For example, ultrasonic instruments may impose a greater risk of generating contaminated aerosols. In addition, dentists should reduce the use of high-speed handpieces and three-way syringes. Negative pressure treatment room/Airborne infection isolation rooms (AIIRs): It is worth noting that patients with suspected or confirmed COVID-19 infection should not be treated in a routine dental practice setting. Instead, this subset of patients should only be treated in negative pressure rooms or AIIRs. Therefore, anticipatory knowledge of health care centers with provision for AIIRs would help dentists to provide emergent dental care if the need arises. Human coronavirus can survive on inanimate surfaces up to 9 days at room temperature, with a greater preference for humid conditions. Therefore, clinic staff should make sure to disinfect inanimate surfaces using chemicals recently approved for COVID-19 and maintain a dry environment to curb the spread of SARS-CoV-2.